Pakistan exports tomato, an important vegetable crop, to different areas of the world and generates valuable revenue. But per hector yield is low compared to other countries. One reason is the inability of tomato varieties to cope with the stresses plant face in the field. Drought is an important stress. Plant breeders, over last fifty years, have used the available genetic diversity to develop improved varieties but have ignored epigenetic diversity. Recently, trangenerational nature of epigenetic modifications especially DNA methylation have been reported meaning that the induced change in the DNA methylation pattern can be transmitted to next generations. Therefore, in the proposed study, the shift in the DNA methylation pattern will be chemically induced and then the plants will be screened for the phenotypes of agronomic interest under drought stress. These plants will be studied phenotypically as well as epi-genotypically to identify the regions where these epi-mutations have occurred and then their transgenerational transmission will be evaluated. The plants with the stable transgenerational epi-mutations will be recommended to the breeders as well as for commercial use subjected to field trials.