Radiation, a substantial element in the environment, has detrimental impact on human health as scientific and technological progression have raised its burden on human beings. Exposure to low levels of radiation has become a common phenomenon. Excessive reactive oxygen radicals (ROS) produced by ionizing radiation (IR) can cause serious oxidative damage, leading to oxidation-reduction (REDOX) system imbalance and immune system damage. Radiation is considered immunosuppressive as damaging normal tissue may cause cancer, death, and loss of neural function in humans and animals. The immune system protects the body from foreign invaders. Immunosuppression is characterized by a series of complex cascade cellular and organ-related events that can result in severe infectious and neoplastic. Here, the immuneboosting effect of honokiol will be studied through a model of immunosuppression in 137 Cs γ irradiated mice. Honokiol is a hydroxylated biphenolic compound, isolated from Magnolia bark, and possess different pharmacological properties such as antioxidant, anti-inflammatory, antibacterial, neuroprotective etc.

The immune-boosting effect of honokiol in irradiated mice will be evaluated by hematological analysis of blood to evaluate the immune cells parameters. The immune organs index and cell counting will be performed for proliferation capability. Tissues oxidative stress biomarkers for lipid peroxidation, enzymatic and non- enzymatic antioxidant levels will be evaluated. Tissue histology of immune organs will be performed to evaluate tissue morphological changes and gene expression analysis to explore the mechanistic potential. To evaluate the immune-boosting capability of honokiol it will be administered to irradiated mice of experimental groups. The objective of this research is to explore the immune-boosting effect of honokiol in radiation-induced immunosuppressed mice.